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KMID : 0545120100200111546
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 11 p.1546 ~ p.1554
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α-Galactosidase from Bacillus megaterium VHM1 and its application in removal of flatulence-causing factors from soymilk
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Aravind Goud G. Patil
Praveen Kumar S. K.
Veerappa H. Mulimani
Yaligara Veeranagouda
Lee Kyoung
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Abstract
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Bacterial strain capable of producing extracellular α-galactosidase was isolated from sugar cane industrial waste soil sample. Microbiological, physiological, and biochemical studies revealed that isolate belonged to Bacillus sp,. Furthermore, 16S rDNA sequence analysis of new isolates was identified as Bacillus megaterium VHM1. The production of α-galactosidase was optimized by various physical culture conditions. Guar gum and yeast extract acted as the best carbon and nitrogen source, respectively for the production of α-galactosidase. The enzyme showed an optimum pH at 7.5 and was stable over a pH between 5 and 9. The enzyme was optimally active in 55˚C and the enzyme was thermostable with half life of 120 minutes at 550C and lost their 90%, residual activity in 120 minutes at 600C. α-Galactosidase was strongly inhibited by Ag2, Cu2, and Hg2+ at 1mM concentration. The metal ions Fe2, Mn2+, and Mg2+ had no effect on α-galactosidase activity, Zn2+,Ni2+, and Ca2+ reduced the enzyme activity slightly. The B megaterium VHM1 enzyme treatment completely hydrolyzed flatulence-causing sugars of soymilk within one and half hour.
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KEYWORD
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Bacillus megaterium, α-Galactosidase, 16S rDNA analysis, Raffinose family, oligosaccharides (RFOs), Soymilk
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